Coding
PPK E173K

Part:BBa_K3583005:Design

Designed by: Sebastian Kusnik   Group: iGEM20_Duesseldorf   (2020-10-22)


Escherichia coli PPK with E173K mutation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1671
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

The mutation was described by Rudat et al in 2018. doi: 10.1128/JB.00697-17

References